Secrets of photosynthesis have been discovered at an atomic level, shedding important new light on this plant super-power that greened the earth more than a billion years ago.
John Innes Centre researchers used an advanced microscopy method called cryo-EM to explore how the photosynthetic proteins are made.
The study, published in Cell, presents a model and resources to stimulate further fundamental discoveries in this field and assist longer term goals of developing more resilient crops.
Dr Michael Webster, group leader and co-author of the paper, said: "Transcription of chloroplast genes is a fundamental step in making the photosynthetic proteins that provide plants with the energy they need to grow. We hope that by understanding this process better – at the detailed molecular level – we will equip researchers looking to develop plants with more robust photosynthetic activity."
"The most important outcome of this work is the creation of a useful resource. Researchers can download our atomic model of the chloroplast polymerase and use it to produce their own hypotheses of how it might function and experimental strategies that would test them."
Photosynthesis takes place inside chloroplasts, small compartments within plant cells that contain their own genome, reflecting their past as free-living photosynthetic bacteria before they were engulfed and co-opted by plants.
The Webster group at the John Innes Centre investigates how plants make photosynthetic proteins, the molecular machines that make this elegant chemical reaction happen, converting atmospheric carbon dioxide and water into simple sugars and producing oxygen as a byproduct. The first stage in protein production is transcription, where a gene is read to produce a 'messenger RNA.' This transcription process is done by an enzyme called RNA polymerase.
It was discovered 50 years ago that chloroplasts contain their own unique RNA polymerase. Since then, scientists have been surprised by how complex this enzyme is. It has more subunits than its ancestor, the bacterial RNA polymerase, and is even bigger than human RNA polymerases.
The Webster group wanted to understand why chloroplasts have such a sophisticated RNA polymerase. To do this, they needed to visualize the structural architecture of the chloroplast RNA polymerase.
The research team used a method called cryogenic electron microscopy (cryo-EM) to image samples of chloroplast RNA polymerase purified from white mustard plants. By processing these images, they were able to build a model that contains the positions of more than 50,000 atoms in the molecular complex.
The RNA polymerase complex comprises 21 subunits encoded in the two genomes, nuclear and chloroplast. Close analysis of this structure, as it performs transcription, allowed the researchers to start explaining these components' functions.
The model allowed them to identify a protein that interacts with the DNA as it is being transcribed and guides it to the enzyme's active site. Another component can interact with the mRNA that is being produced that likely protects it from proteins that would degrade it before it is translated into protein. Dr Webster said: "We know that each component of the chloroplast RNA polymerase has a vital role because plants that lack any one of them cannot make photosynthetic proteins and consequently cannot turn green. We are studying the atomic models carefully to pinpoint what the role is for each of the 21 components of the assembly."
Source: jic.ac.uk