Agdia has launched a new product for rapid, in-field detection of Orchid fleck virus (OFV) on its ImmunoStrip platform.
Orchid fleck virus is an emerging Dichorhavirus that is transmitted by Brevipalpus mites (also known as flat mites or false spider mites).
First reported in Japan, OFV has now been detected across the globe, with reports of detection out of China, Europe, South Africa, Australia, and the Americas.
Orchid fleck virus has been found to infect at least 70 orchid species from many genera, including Dendrobium, Cymbidium, Epidendrum, Dendrochilum (syn. Coelogyne), and Brassia (spider orchids).
Orchid fleck virus is thought to be one of the causal agents of citrus leprosis disease that infects oranges, lime, grapefruit and lemon. Symptoms of citrus leprosis disease include lesions on the leaves, twigs, branches and fruit of citrus trees.
Other known hosts of OFV include Liriope spp. (Lilyturf), Veronica (Speedwell), Aspidistra elatior (cast iron plant/bar-room plant), Cordyline fructicosa (Ti), and Ophiopogon intermedius (Aztec grass).
In orchids, symptoms of Orchid fleck virus infection include chlorotic and necrotic flecks, spots and ringspots. The virus may stay localized to the lesions or spread to other areas of the plant.
This test was developed not only due to increasing official reports of OFV around the world but also because many concerned friends and clients of Agdia in the orchid community reached out to request an onsite testing solution. For cultivators interested in sending samples to Agdia's service lab for testing, Orchid fleck virus is now included on Agdia Testing Services Orchid and Citrus screens.
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The new ImmunoStrip for Orchid fleck virus detected all 27 OFV-positive samples tested during product validation, resulting in a diagnostic sensitivity of 100%. No cross-reactions were observed when this product was tested against other virus species. No false positive reactions were observed after testing against 27 healthy host species tissues.
Agdia's ImmunoStrip platform provides end-users with utility; samples can be tested in the field or lab by those with no previous diagnostic experience, and results are visualized within 30 minutes. This product includes everything necessary to perform a test and does not require special equipment.
The testing protocol is simple and involves three steps: sample collection and extraction in Agdia buffer bags, placing the ImmunoStrip in the sample extraction bag, and allowing the results to develop.
Test results are visualized as a single control line or a control and test line for negative or positive results, respectively. The introduction of Agdia's ImmunoStrip for Orchid fleck virus expands their catalog to 56 plant pathogen products on this platform.
The OFV ImmunoStrip is sold in kits of 5 or 25 strips, and kits include everything necessary to perform a test. Agdia provides a one-year warranty and comprehensive customer support on all purchased products.
For more information:
Agd
ia
52642 County Road 1
Elkhart, IN 46514
phone 1-574-264-2615
fax 1-574-264-2153
info@agdia.com
www.agdia.com